human dermal fibroblast cells  (ATCC)

Journal: Plants

Article Title: Ginsenoside-Enriched Panax ginseng Sprouts Cultivated from Aquaponic System with a Novel Nutrient Solution Regulate LPS-Induced Inflammatory Cytokines and UVB-Induced Photoaging Responses via MAPK/AP-1 Signaling Pathways

doi: 10.3390/plants14111712

Figure Lengend Snippet: Effects of ginseng extracts (GE), the extract of kelp fermentates−treated ginseng (FGE), the water fraction of crude saponin extract from FGE (WFGE) and the 70% ethanol fraction of crude saponin extract from FGE (EFGE) on cell viability ( A , B ), procollagen ( C ) and MMP−1 ( D ), and the expressions of MMP−1 and MMP−3 ( E ) in UVB−irradiated Hs68 cells. Data values are expressed as mean as S.D. of triplicate determinations. Significant differences were compared with control at * p < 0.05, ** p < 0.01, and *** p < 0.001, and with the UVB group at ## p < 0.01 and ### p < 0.001 by one−way analysis of variance and Tukey’s multiple comparison.

Article Snippet: Mouse macrophage RAW264.7 cells and human dermal fibroblast Hs68 cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Irradiation, Control, Comparison

Journal: Plants

Article Title: Ginsenoside-Enriched Panax ginseng Sprouts Cultivated from Aquaponic System with a Novel Nutrient Solution Regulate LPS-Induced Inflammatory Cytokines and UVB-Induced Photoaging Responses via MAPK/AP-1 Signaling Pathways

doi: 10.3390/plants14111712

Figure Lengend Snippet: Effects of ginseng extracts (GE), the extract of kelp fermentates−treated ginseng (FGE), the water fraction of crude saponin extract from FGE (WFGE) and the 70% ethanol fraction of crude saponin extract from FGE (EFGE) on expressions of mitogen−activated protein kinases ( A ) and activator protein−1 ( B ) signaling pathways in UVB−irradiated Hs68 cells. Data values are expressed as mean as S.D. of triplicate determinations. Significant differences were compared with control at * p < 0.05, ** p < 0.01, and *** p < 0.001 and with UVB group at ### p < 0.001 by one−way analysis of variance and Tukey’s multiple comparison.

Article Snippet: Mouse macrophage RAW264.7 cells and human dermal fibroblast Hs68 cells were obtained from the American Type Culture Collection (ATCC; Rockville, MD, USA).

Techniques: Protein-Protein interactions, Irradiation, Control, Comparison

Journal: Antioxidants

Article Title: 10(E)-Pentadecenoic Acid Inhibits Melanogenesis Partly Through Suppressing the Intracellular MITF/Tyrosinase Axis

doi: 10.3390/antiox13121547

Figure Lengend Snippet: Effects of 10E-PDA on inhibiting melanogenesis in α-MSH-stimulated B16F10 melanoma cells. ( a ) B16F10, ( b ) HS68, and ( c ) HaCaT cells were treated with increasing concentrations of 10E-PDA (1–100 μM) for 24 h, after which cell viability was assessed (n = 4 per group). ( d , e ) B16F10 cells were pretreated with different concentrations of 10E-PDA (1–15 μM) or kojic acid (30 μM) for 1 h, followed by exposure to α-MSH (500 nM) for 6 days to measure melanin content ( d ) or for 3 days to examine tyrosinase activity ( e ). In both panels, the white bar represents the untreated control group without α-MSH stimulation, the black bar represents the α-MSH-stimulated control group, and the dark gray bars indicate the 10E-PDA-treated groups under α-MSH stimulation. In panel ( e ), the light gray bar represents the positive control group treated with kojic acid under α-MSH stimulation. Data are shown as mean ± SEM. ### p < 0.001 compared with the untreated control group, and * p < 0.05, ** p < 0.01, *** p < 0.001 compared with the α-MSH-treated group.

Article Snippet: The B16F10 murine melanoma cell line and Hs68 human dermal fibroblast cell line were purchased from the American Type Culture Collection (ATCC, Rockville, MD, USA).

Techniques: Activity Assay, Control, Positive Control

Journal: Biochemistry and Biophysics Reports

Article Title: Piceatannol enhances hyaluronic acid synthesis through SIRT1-Mediated HAS2 upregulation in human dermal fibroblasts

doi: 10.1016/j.bbrep.2024.101746

Figure Lengend Snippet: Effects of PIC on HAS2 and HYAL2 Expression in Human Dermal Fibroblasts Hs68 cells were subjected to treatment with either DMSO control (CTRL) or 20 μM piceatannol (PIC) for 16 h. Subsequently, mRNA expression levels of (A) HAS2 and (B) HYAL2 were assessed via quantitative real-time PCR and normalized to GAPDH expression. Data are presented as the mean ± standard deviation (n = 3). * p < 0.05 vs CTRL (Student's t-test).

Article Snippet: Human dermal fibroblast Hs68 cells (JCRB Cell Bank, Japan) were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, USA) supplemented with 10 % fetal bovine serum (FBS, Global Life Sciences Technologies Japan K·K., Japan) and 1 % penicillin-streptomycin (Gibco, USA) at 37 °C in a 5 % CO 2 environment.

Techniques: Expressing, Control, Real-time Polymerase Chain Reaction, Standard Deviation

Journal: Biochemistry and Biophysics Reports

Article Title: Piceatannol enhances hyaluronic acid synthesis through SIRT1-Mediated HAS2 upregulation in human dermal fibroblasts

doi: 10.1016/j.bbrep.2024.101746

Figure Lengend Snippet: Effects of SIRT1 Inhibitor on PIC-Induced Hyaluronic Acid Biosynthesis-Related Gene Expression Hs68 cells were subjected to treatment with or without 10 μM Ex527 for 6 h, followed by treatment with 20 μM PIC and incubation for 16 h. Subsequently, gene expression levels of (A) HAS2 and (B) HYAL2 were assessed via quantitative real-time PCR and normalized to GAPDH expression. Data are presented as mean ± standard deviation (n = 6). Different letters indicate significance ( p < 0.05, Tukey's test).

Article Snippet: Human dermal fibroblast Hs68 cells (JCRB Cell Bank, Japan) were cultured in Dulbecco's modified Eagle's medium (DMEM, Gibco, USA) supplemented with 10 % fetal bovine serum (FBS, Global Life Sciences Technologies Japan K·K., Japan) and 1 % penicillin-streptomycin (Gibco, USA) at 37 °C in a 5 % CO 2 environment.

Techniques: Gene Expression, Incubation, Real-time Polymerase Chain Reaction, Expressing, Standard Deviation